CHROMOSOME MICRODISSECTION AND MICROCLONING PDF

Chromosome microdissection is a technique that physically removes a large section of DNA from a complete chromosome. The smallest portion of DNA that can be isolated using this method comprises 10 million base pairs - hundreds or thousands of individual genes. Scientists who study chromosomes are known as cytogeneticists. They are able to identify each chromosome based on its unique pattern of dark and light bands. Certain abnormalities, however, cause chromosomes to have unusual banding patterns. For example, one chromosome may have a piece of another chromosome inserted within it, creating extra bands.

Author:Kisar Moogudal
Country:Italy
Language:English (Spanish)
Genre:Life
Published (Last):28 September 2005
Pages:308
PDF File Size:4.54 Mb
ePub File Size:5.24 Mb
ISBN:453-9-37332-825-4
Downloads:5563
Price:Free* [*Free Regsitration Required]
Uploader:Akinomi



Protocol DOI: Chromosome microdissection followed by microcloning is an efficient tool combining cytogenetics and molecular genetics that can be used for the construction of the high density molecular marker linkage map and fine physical map, the generation of.

Chromosome microdissection followed by microcloning is an efficient tool combining cytogenetics and molecular genetics that can be used for the construction of the high density molecular marker linkage map and fine physical map, the generation of probes for chromosome painting, and the localization and cloning of important genes. Here, we describe a modified technique to microdissect a single chromosome, paint individual chromosomes, and construct single-chromosome DNA libraries.

Degenerated oligonucleotide-primed-PCR, Single-chromosome library. Antibody Data Search Beta. Authors: Ying-Xin Zhang 1 , 2 ,. Chuan-Liang Deng 3 ,. Zan-Min Hu 1. Ying-Xin Zhang 1 , 2 ,. Full text PDF Related articles. Citations 3 Recent citations: Tomohiro Yanagi et al.

Related articles Based on techniques. Wang , , Springer Protocols See more. Genome — Zhou RN, Hu ZM The development of chromosome microdissection and microcloning technique and its applications in genomic research. Genome —76 Han FP, Gao Z, Birchler JA Reactivation of an inactive centromere reveals epigenetic and structural components for centromere specification in maize.

Plant Cell —

THE EYE OF REVELATION BY PETER KELDER PDF

The Chromosome Microdissection and Microcloning Technique

Chromosome microdissection followed by microcloning is an efficient tool combining cytogenetics and molecular genetics that can be used for the construction of the high density molecular marker linkage map and fine physical map, the generation of probes for chromosome painting, and the localization and cloning of important genes. Here, we describe a modified technique to microdissect a single chromosome, paint individual chromosomes, and construct single-chromosome DNA libraries. Keywords: Chromosome microdissection and microcloning; Degenerated oligonucleotide-primed-PCR; Fluorescence in situ hybridization; Single-chromosome library. This site needs JavaScript to work properly. Please enable it to take advantage of the complete set of features! Clipboard, Search History, and several other advanced features are temporarily unavailable.

THE UNFOLDING DRAMA OF REDEMPTION PDF

Chromosome microdissection

Protocol DOI: Chromosome microdissection followed by microcloning is an efficient tool combining cytogenetics and molecular genetics that can be used for the construction of the high density molecular marker linkage map and fine physical map, the generation of. Chromosome microdissection followed by microcloning is an efficient tool combining cytogenetics and molecular genetics that can be used for the construction of the high density molecular marker linkage map and fine physical map, the generation of probes for chromosome painting, and the localization and cloning of important genes. Here, we describe a modified technique to microdissect a single chromosome, paint individual chromosomes, and construct single-chromosome DNA libraries. Degenerated oligonucleotide-primed-PCR, Single-chromosome library. Antibody Data Search Beta.

8VM533M-RZ MANUAL PDF

Plant Cytogenetics pp Cite as. Chromosome microdissection followed by microcloning is an efficient tool combining cytogenetics and molecular genetics that can be used for the construction of the high density molecular marker linkage map and fine physical map, the generation of probes for chromosome painting, and the localization and cloning of important genes. Here, we describe a modified technique to microdissect a single chromosome, paint individual chromosomes, and construct single-chromosome DNA libraries. Springer Nature is developing a new tool to find and evaluate Protocols. Learn more.

Related Articles